Costa Rica Research Articles

Understanding the factors that shape vaccination ecosystem resilience: a qualitative assessment of international expert experiences and perspectives

Introduction‘Shocks’ or external stressors to vaccination programmes can lead to decreased vaccination coverage rates. The capacity of vaccination ecosystems to effectively respond and adapt to shocks demonstrates programme resilience. This...

Development, implementation, and evaluation of the virtual platform Quimieduca as a didactic strategy for the teaching of Chemistry in high school

The evolution of teaching-learning processes and technologies has driven the use of computer tools to support education. Their use in science teaching promotes the development of scientific and technological skills. In the present research work, a virtual platform called Quimieduca was designed, implemented, and evaluated, with resources and didactic activities as an alternative teaching strategy for the secondary school teaching of Chemistry in Costa Rica. An initial diagnosis was carried out to determine the need and feasibility of implementing the platform. Four key topics from the Chemistry Program of the Ministry of Public Education were selected to develop the resources and didactic activities on the platform. It was designed and programmed using the Edmodo virtual platform.  The platform was implemented with tenth-year students from Colegio Gravilias during the 2022 school year. Two classes (10-3 and 10-4) utilized the Quimieduca platform, while the other two classes (10-1 and 10-2) formed the control group without access to the tool. The effectiveness of Quimieduca was evaluated both from the perspective of teachers and through the indicators of students’ academic achievements. Teachers rated the platform positively in terms of its form, substance, and design aspects. Furthermore, the results demonstrated that the group of students who used the platform obtained higher performance indices, suggesting that the use of the platform facilitated a more significant advancement in the learning of the assessed indicators.

Ocean Variability in the Costa Rica Thermal Dome Region from 2012 to 2021

Satellite ocean color and sea surface temperature (SST) observations from 2012 to 2021 and sea surface salinity (SSS) measurements from the Soil Moisture Active Passive (SMAP) mission from 2015 to 2021 are used to characterize and quantify the seasonal and interannual variability in the physical, optical, and biological sea surface features in the Costa Rica Thermal Dome (CRTD) region. High-resolution climatology and the seasonal variability in SST, SSS, and ocean color properties are produced. Chlorophyll-a (Chl-a) concentration, SST, and SSS show these three properties are linked with similar spatial patterns and seasonal variations, i.e., elevated Chl-a concentrations, match the depressed SST and increased SSS and vice versa. This reflects that the physical driving force is the same for these three ocean properties and implies that nutrient supply associated with the physical processes is the major driver for the seasonal biological variability. The interannual changes in Chl-a, SST, and SSS also show that these three ocean properties are consistent among themselves. The positive (negative) Chl-a anomaly generally occurs with negative (positive) SST anomaly and enhanced (reduced) SSS. The in situ measurements evidently show that the subsurface ocean dynamics in the upper 100 m controls the sea surface variability for Chl-a, SST, and SSS. We report that no significant enhancement of Chl-a is observed in the CRTD region during the central Pacific (CP)-type 2020–2021 La Niña event, while Chl-a changes are significant in the other three of four ENSO events between 2012 and 2021. Furthermore, the difference in Chl-a variability driven by the CP-type ENSO and eastern Pacific (EP)-type ENSO is further discussed.

Substitution Patterns of the English Voiced Interdental Fricative by L1 Costa Rican Spanish Speakers

L1 Spanish speakers learning English struggle to produce the English voiced interdental fricative [ð]. This is surprising as [ð] occurs naturally in Spanish as an intervocalic allophone of /d/. To investigate Spanish speakers’ production of [ð] in English, I conducted a study with ten L1 Costa Rican Spanish/L2 English speakers. The results show that English voiced interdental fricatives were produced with target pronunciation 43.4% (214/494) of the time and were substituted in the remaining 56.6% (280/494). It was determined that target-sound substitution patterns depend on the phonological contexts in which the target sound occurs (word-initial vs. intervocalic), and the type of speaking task (reading vs. spontaneous). I interpret these results as partially due to phonological transfer: L1 allophonic information affects L2 speech production. The results also show that the realization of the segments is affected by the different experimental tasks: speakers have more target-like production in tasks where there is an opportunity for more careful pronunciation (reading task) than in more spontaneous ones. Finally, I argue that the unexpected appearance of the alveolar tap [ɾ] is due to an interaction between the English /d/ tapping allophone rule and the status of [d] and [ð] in the learner’s interlanguage.

Rice yield in Costa Rican Central Pacific did not improve with a urease inhibitor

Urea is widely used as nitrogen (N) source for rice fertilization in Costa Rica, despite its low efficiency linked to ammonia losses. To assess urea management alternatives, two field experiments were conducted in the Central Pacific region of Costa Rica to study the effect of N-(n-butyl) thiophosphoric triamide (NBPT) on rice yield and N use efficiency (NUE). In Experiment 1 (Exp1) three tillage treatments (commercial-CT-, reduced-RT-, and reduced tillage with previous subsoiler-RTS-) were evaluated with three N managements: control (without N), urea at 124 kg N ha-1 with and without NBPT. In Experiment 2 (Exp2), a 100 kg N ha-1 rate (with and without NPBT) was evaluated along with a control (without N). NUE was estimated using 15N urea isotopic labeling technique for both trials. In Exp1, a significant difference of 4.8% in NUE for grain was observed among urea with and without NBPT, but no tillage effect was observed. No statistically significant differences were observed in yield among the fertilization treatments (Exp1: 3.56 ± 0.98 t ha-1 for urea and 3.85 ± 0.85 t ha-1 for urea with NBPT; Exp2: 3.38 ± 0.39 t ha-1 for urea and 3.40 ± 0.58 t ha-1 for urea with NBPT) or due to different tillage practices (CT: 3.33 ± 0.79 t ha-1, RT: 3.56 ± 0.74 t ha-1, and RTS: 4.23 ± 0.98 t ha-1). Although the NBPT is a viable option to reduce ammonia losses, its adoption in tropical conditions might be restricted by the small impact on yield.

The Influence of Individualism and Collectivism on Costa Rican Children, Adolescents and Young adults’ Decisions Facing Inequality in Resource Distribution

ABSTRACT We examined how induced cultural orientation (individualist or collectivist) influences decisions in resource distribution in middle childhood (N = 166, M age = 9.85, SD = 0.96, females = 51.5%), early adolescence (N = 254, M age = 13.42, SD = 0.72, females = 50.8%) and young adults (N = 338, M age = 21.41, SD = 3.77, females = 46.4%), among Costa Rican samples. We found influence of induced individualism in rejection of highly unfair offers in the ultimatum game (9:1) in the three age groups and to a lesser degree among adolescents compared to children and adults.

Book review: Living with algorithms: Agency and user culture in Costa Rica

Book review: Living with algorithms: Agency and user culture in Costa Rica

Resistant Escherichia coli isolated from wild mammals from two rescue and rehabilitation centers in Costa Rica: characterization and public health relevance

This study aimed to characterize the antimicrobial resistance (AMR) and virulence profiles of 67 Escherichia coli isolates obtained from faecal samples of 77 wild mammals from 19 different species, admitted in two rescue and rehabilitation centers in Costa Rica. It was possible to classify 48% (n = 32) of the isolates as multidrug-resistant, and while the highest resistance levels were found towards commonly prescribed antimicrobials, resistance to fluoroquinolones and third generation cephalosporins were also observed. Isolates obtained from samples of rehabilitated animals or animals treated with antibiotics were found to have significantly higher AMR levels, with the former also having a significant association with a multidrug-resistance profile. Additionally, the isolates displayed the capacity to produce α-haemolysins (n = 64, 96%), biofilms (n = 51, 76%) and protease (n = 21, 31%). Our results showed that AMR might be a widespread phenomenon within Costa Rican wildlife and that both free-ranging and rehabilitated wild mammals are potential carriers of bacteria with important resistance and virulence profiles. These results highlight the need to study potential sources of resistance determinants to wildlife, and to determine if wild animals can disseminate resistant bacteria in the environment, potentially posing a significant threat to public health and hindering the implementation of a “One Health” approach.

Applying Community-Based Participatory Research Principles to Global Oral Health: A Case Study from Costa Rica

Applying Community-Based Participatory Research Principles to Global Oral Health: A Case Study from Costa Rica

The mediating role of marketing management in the relationship between online presence and product innovation among SMEs

PurposeThis study explores the mediating role of marketing management in the relationship between online presence and product innovation among Small and Medium Enterprises (SMEs).Design/methodology/approachThe sample comprises 205 Costa Rican SMEs collected by the Global Competitiveness Project during the first half of 2019. The data were analyzed using a two-stage modeling strategy for ordinary regression models to analyze mediation effects.FindingsMarketing management as a strategic resource or capability accounts for the relationship between online presence and product innovation performance in SMEs, meaning that online presence resources require complementary organizational capabilities in marketing management to enhance product innovation.Originality/valueThis study, grounded in the resource-based view theory, contributes to the innovation field by identifying marketing management capabilities as an intermediate strategic interaction between online presence and product innovation performance in SMEs. Thus, managers should recognize the advantages of integrating marketing management principles and tactics into online presence tools to realize the value of their products by tailoring them to their client’s needs.

Quantifying movement of the coffee berry borer at the interface between coffee plantations and adjacent land uses

IntroductionInsect pests cause important crop production losses worldwide. Their distribution and movement are affected by climate and land use change and agriculture intensification. Site colonization by insect pests is dependent on pest dispersal capability, the availability of resources, the presence of competitors or predators, the weather conditions and the characteristics of the surrounding landscape. Movement of pests between the plots might be considered in pest management strategies to counterbalance the traditional plot oriented strategies. In this study, our objective was to provide evidence of the movement of the coffee berry borer (CBB), the most important pest in coffee cultivation, from neighboring coffee plantations to adjacent land uses at different time periods of the coffee production cycle.MethodsFor 10 months we captured the CBB with funnel traps in 13 coffee plots that had an interface with forests, pastures, and abandoned coffee plantations in Costa Rica. At each interface, we established three transects with a minimum distance of 50 m between them, in the direction of the wind. Within each transect, we placed four traps 20 m apart. We fitted generalized linear mixed models to evaluate the relationship between CBB captures and the type of interface, the position of the trap, wind velocity, rainfall, temperature and relative humidity, and their interactions.ResultsOur findings suggest that CBB moves into adjacent land uses when the coffee resource in the plot is limited. This effect varies according to the interface and the position of the trap. We also found an interaction between the interface and the position of the trap with the wind and relative humidity.DiscussionOur findings suggest that movement of the CBB partly depends upon the adjacent land uses. The forest creates a barrier to CBB movement and may prevent the transport of the CBB considering the action of the wind speed. The pasture may facilitate movement of the CBB through the action of the wind speed and infest coffee plantations beyond its dispersal capacity. Our results support the importance of considering the landscape context when developing CBB management strategies.

Knowledge and attitudes toward anaphylaxis to local anesthetics in dental practice

Objective/aimThe absence of a comprehensive understanding of potential anaphylactic reactions to local anesthetics (LAs) and management can result in grave consequences. For this reason we aim to assess Latin American dentists’ knowledge, preparedness, and competency in managing anaphylactic reactions to LAs.Materials and MethodsDesign: A cross-sectional study was conducted from November 2021 to February 2022. Board-certified dentists answered a survey comprising 26 structured questions. Chi-square tests and logistic regression models were performed in Stata 17.0. Setting: Argentina, Brazil, Colombia, Costa Rica, Ecuador, Honduras, Mexico, Peru, Venezuela, and other Latin American countries.ResultsOf 507 respondents, lidocaine was the most frequently used LA (88.1%). While 85.2% could identify dyspnea as a symptom of anaphylaxis, only 50.1% knew the correct route for epinephrine administration, and just 43.5% had epinephrine in their emergency kits. Confidence in managing anaphylactic reactions was low (9.6%). Older age was inversely related to both knowledge of anaphylaxis management and the possession of epinephrine (P = 0.003 and P = 0.0001, respectively).DiscussionOur study highlights a concerning discrepancy between the practical readiness of Latin American dentists in handling anaphylaxis.ConclusionThe study’s findings underscore the need for educational interventions to improve the readiness to identify and handle anaphylactic emergencies in dental practice.

Healthy Diets and Lifestyles in the World: Mediterranean and Blue Zone People Live Longer. Special Focus on Gut Microbiota and Some Food Components.

Longevity has been associated with healthy lifestyles, including some dietary regimens, such as the Mediterranean diet (MedDiet) and the Blue Zone (BZ) diets. MedDiet relies on a large consumption of fruit, vegetables, cereals, and extra-virgin olive oil, with less red meat and fat intake. Four major BZ have been recognized in the world, namely, Ogliastra in Sardinia (Italy), Ikaria (Greece), the Peninsula of Nicoya (Costa Rica), and Okinawa (Japan). Extreme longevity in these areas has been associated with correct lifestyles and dietary regimens. Fibers, polyphenols, beta-glucans, and unsaturated fatty acids represent the major constituents of both MedDiet and BZ diets, given their anti-inflammatory and antioxidant activities. Particularly, inhibition of the NF-kB pathway, with a reduced release of pro-inflammatory cytokines, and induction of T regulatory cells, with the production of the anti-inflammatory cytokine, interleukin- 10, are the main mechanisms that prevent or attenuate the "inflammaging." Notably, consistent physical activity, intense social interactions, and an optimistic attitude contribute to longevity in BZD areas. Commonalities and differences between MedDIet and BZ diets will be outlined, with special reference to microbiota and food components, which may contribute to longevity.

Pesticide exposure, birth size, and gestational age in the ISA birth cohort, Costa Rica.

To examine associations of prenatal biomarkers of pesticide exposure with birth size measures and length of gestation among newborns from the Infants' Environmental Health (ISA) birth cohort, Costa Rica. We included 386 singleton liveborn newborns with data on birth size measures, length of gestation, and maternal urinary biomarkers of chlorpyrifos, synthetic pyrethroids, mancozeb, pyrimethanil, and 2, 4-D during pregnancy. We associated biomarkers of exposure with birth outcomes using multivariate linear regression and generalized additive models. Concentrations were highest for ethylene thiourea (ETU, metabolite of mancozeb), median = 3.40; p10-90 = 1.90-6.79 µg/L, followed by 3,5,6-trichloro-2-pyridinol (TCP, metabolite of chlorpyrifos) p50 = 1.76 p10-90 = 0.97-4.36 µg/L, and lowest for 2,4-D (p50 = 0.33 p10-90 = 0.18-1.07 µg/L). Among term newborns (≥37 weeks), higher prenatal TCP was associated with lower birth weight and smaller head circumference (e.g., β per 10-fold-increase) during the second half of pregnancy = -129.6 (95% confidence interval [CI] = -255.8, -3.5) grams, and -0.61 (95% CI = -1.05, -0.17) centimeters, respectively. Also, among term newborns, prenatal 2,4-D was associated with lower birth weight (β per 10-fold-increase = -125.1; 95% CI = -228.8, -21.5), smaller head circumference (β = -0.41; 95% CI = -0.78, -0.03), and, during the second half of pregnancy, with shorter body length (β = -0.58; 95% CI = -1.09, -0.07). Furthermore, ETU was nonlinearly associated with head circumference during the second half of pregnancy. Biomarkers of pyrethroids and pyrimethanil were not associated with birth size, and none of the biomarkers explained the length of gestation. Prenatal exposure to chlorpyrifos and 2,4-D, and, possibly, mancozeb/ETU, may impair fetal growth.

Unearthing care: Rooting alternative agricultural practices in Norway and Costa Rica

This article contributes to the growing literature on human-soil relations by exploring how care for agricultural soils unfolds among farmers who engage with alternative agricultural practices across different productions and sites in Norway and Costa Rica. These farmers approach soil as a living being and seek to approach care with macro- and microorganisms in response to soil challenges and economic instabilities. The article follows recent literature on soil care in showing how agricultural practices challenge the dominant approach to soils as passive. However, the article argues the necessity of expanding on existing notions of care. This, I argue, involves ethnographically “unearthing” care: unpacking and situating a diversity of soil care practices, their human and other-than-human entanglements, and how these relations are conditioned by environmental, genealogical, sociocultural, temporal, epistemic, economic, and political mechanisms within and beyond the farm. Considering these variables is essential to keep soil care in the emerging literature from following a romanticizing path toward abstract individualism.

MORPHOLOGICAL AND MOLECULAR CHARACTERIZATION OF AMBLYOMMA SCUTATUM (ACARI: IXODIDAE) ACCIDENTALLY INTRODUCED IN ITALY.

Eight ticks were found in Comacchio (FE), Italy parasitizing a young black iguana (Ctenosaura similis) that had been accidentally transported in a commercial plant container from Costa Rica. Specimens were identified morphologically as Amblyomma scutatum and then confirmed by the barcoding of the mitochondrial cytochrome c oxidase subunit 1 gene. Amblyomma scutatum is a common tick known to infest reptiles in Central America, Mexico, and Venezuela, but not in Europe. In Italy, the possibility for this tick to become endemic is unlikely because of the absence of its principal hosts. Nevertheless, this finding confirms the high risk of introducing exotic species that is linked with global commerce and therefore the need for veterinary control of shipments.

International Nosocomial Infection Control Consortium (INICC) report of healthcare-associated infections, data summary of 25 countries for 2014-2023, Surgical Site Infections Module

BackgroundSurgical site infection (SSI) prevalence is higher in low and middle-income countries (LMICs) than in high-income counterparts. This study covers 116 INICC member hospitals in 75 cities across 25 Latin American, Asian, Eastern European, and Middle Eastern countries, including Argentina, Bahrain, Brazil, Colombia, Costa Rica, Dominican Republic, Ecuador, Egypt, Honduras, India, Kosovo, Kuwait, Lebanon, Mexico, Mongolia, Pakistan, Papua New Guinea, Philippines, Poland, Romania, Saudi Arabia, Thailand, Turkey, Venezuela, and Vietnam. MethodsProspective cohort multinational surveillance data were collected through the INICC Surveillance Online System. CDC-NHSN definitions were applied for surgical site infections (SSI). Surgical procedures were categorized into 41 types according to the ICD-9 criteria, 9th edition. ResultsFrom 2014 to 2023, we collected data on 1,251 SSIs associated with 56,617 SPs. SSI rates were significantly higher in SPs of INICC compared to CDC-NSHN data, including hip prosthesis (3.68% vs. 0.67%, RR=5.46, 95%CI=3.71-8.03, p<0.0001), knee prosthesis (2.02% vs. 0.58%, RR=3.49, 95%CI=1.87-6.49, p<0.0001), coronary artery bypass graft (4.16% vs. 1.37%, RR=3.03, 95%CI=2.35-3.91, p<0.0001, peripheral vascular bypass (15.69% vs. 2.93%, RR=5.35, 95%CI=2.30-12.48, p<0.0001), abdominal aortic aneurysm repair (8.51% vs. 2.12%, RR=4.02, 95%CI=2.11-7.65, p<0.0001), spinal fusion (6.47% vs. 0.70%, RR=9.27, 95%CI=6.21-13.84, p<0.0001), and laminectomy (2.68% vs. 0.72%, RR=3.75, 95%CI=2.36-5.95, p<0.0001), among others. ConclusionsElevated SSI rates in LMICs emphasize the need for effective interventions to alleviate this substantial burden.

Hunting habits die hard: Conserved prey preferences in army ants across two distant neotropical rainforests

AbstractArmy ants are widely recognized as keystone species in neotropical rainforests due to their role as important arthropod predators. Their large‐scale raids involve thousands of workers scouring the forest floor in pursuit of prey, primarily capturing other invertebrates. Up to 20 species of army ants coexist in a rainforest, and dietary niche differentiation has been proposed as a mechanism to alleviate competition among them. Based on only a handful of study sites, however, our understanding of the precise dietary preferences and the extent of niche differentiation remains notably limited. In this study, we aimed to expand our knowledge of army ant communities by resolving an Ecuadorian predation network consisting of 244 prey species and 13 army ant species representing the five known neotropical army ant genera: Cheliomyrmex, Eciton, Labidus, Neivamyrmex, and Nomamyrmex. We collected 2156 prey items from 180 army ant raids/emigrations, and of these, we identified 1945 prey items to the family level, 1313 to the genus level, and 664 to the species level based on morphological identifications and DNA barcodes. Prey consisted primarily of other ants (1843 prey items; 153 ant species), to the largest part ant brood (N = 1726). Hence, most army ant species chiefly plundered the nests of other ants, while the three swarm raiding species, that is, Lab. praedator, Lab. spininodis, and Ec. burchellii, exhibited a relatively high proportion of non‐ant invertebrate prey in their diet. The predation network showed a high degree of specialization (H2′ = 0.65), characterized by little dietary niche overlap among sympatric species. We compared the Ecuadorian network with one previously studied in Costa Rica and found that, despite the large geographic distance, prey preferences remained remarkably similar. We discovered species‐specific preferences for captured ant genera and species, despite some species turnover in both army ants and prey. Additionally, army ants also exhibited consistent spatiotemporal raiding preferences across study sites. In conclusion, predation preferences within army ant communities exhibited consistency in multiple niche dimensions across two distant neotropical rainforests, suggesting a notable level of predictability within army ant predation networks.

Relationships Between Plankton Size Spectra, Net Primary Production, and the Biological Carbon Pump

AbstractPhotosynthesis in the surface ocean and subsequent export of a fraction of this fixed carbon leads to carbon dioxide sequestration in the deep ocean. Ecological relationships among plankton functional groups and theoretical relationships between particle size and sinking rate suggest that carbon export from the euphotic zone is more efficient when communities are dominated by large organisms. However, this hypothesis has never been tested against measured size spectra spanning the &gt;5 orders of magnitude found in plankton communities. Using data from five ocean regions (California Current Ecosystem, North Pacific subtropical gyre, Costa Rica Dome, Gulf of Mexico, and Southern Ocean subtropical front), we quantified carbon‐based plankton size spectra from heterotrophic bacteria to metazoan zooplankton (size class cutoffs varied slightly between regions) and their relationship to net primary production and sinking particle flux. Slopes of the normalized biomass size spectra (NBSS) varied from −1.6 to −1.2 (median slope of −1.4 equates to large 1–10 mm organisms having a biomass equal to only 7.6% of the biomass in small 1–10 μm organisms). Net primary production was positively correlated with the NBSS slope, with a particularly strong relationship in the microbial portion of the size spectra. While organic carbon export co‐varied with NBSS slope, we found only weak evidence that export efficiency is related to plankton community size spectra. Multi‐variate statistical analysis suggested that properties of the NBSS added no explanatory power over chlorophyll, primary production, and temperature. Rather, the results suggest that both plankton size spectra and carbon export increase with increasing system productivity.

Metabolite signatures associated with microRNA miR-143-3p serve as drivers of poor lung function trajectories in childhood asthma

Lung function trajectories (LFTs) have been shown to be an important measure of long-term health in asthma. While there is a growing body of metabolomic studies on asthma status and other phenotypes, there are no prospective studies of the relationship between metabolomics and LFTs or their genomic determinants. We utilized ordinal logistic regression to identify plasma metabolite principal components associated with four previously-published LFTs in children from the Childhood Asthma Management Program (CAMP) (n=660). The top significant metabolite principal component (PCLF) was evaluated in an independent cross-sectional child cohort, the Genetic Epidemiology of Asthma in Costa Rica Study (GACRS) (n=1151) and evaluated for association with spirometric measures. Using meta-analysis of CAMP and GACRS, we identified associations between PCLF and microRNA, and SNPs in their target genes. Statistical significance was determined using an false discovery rate-adjusted Q-value. The top metabolite principal component, PCLF, was significantly associated with better LFTs after multiple-testing correction (Q-value=0.03). PCLF is composed of the urea cycle, caffeine, corticosteroid, carnitine, and potential microbial (secondary bile acid, tryptophan, linoleate, histidine metabolism) metabolites. Higher levels of PCLF were also associated with increases in lung function measures and decreased circulating neutrophil percentage in both CAMP and GACRS. PCLF was also significantly associated with microRNA miR-143-3p, and SNPs in three miR-143-3p target genes; CCZ1 (P-value=2.6×10-5), SLC8A1 (P-value=3.9×10-5); and TENM4 (P-value=4.9×10-5). This study reveals associations between metabolites, miR-143-3p and LFTs in children with asthma, offering insights into asthma physiology and possible interventions to enhance lung function and long-term health. Molecular data for CAMP and GACRS via the Trans-Omics in Precision Medicine (TOPMed) program was supported by the National Heart, Lung, and Blood Institute (NHLBI).